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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
Thiazolyl Blue Tetrazolium Bromide
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
1G
噻唑蓝
Thiazolyl Blue Tetrazolium Bromide
98%
产品名称
Frequently Asked Questions
Live Chat and Frequently Asked Questions
are available for this Product.
包装
1, 5, 10 g in glass bottle
100, 250, 500 mg in glass bottle
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
性质
| Related Categories | Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Core Bioreagents, Fluorescent reagents for cell counting, Reagents, Research Essentials, | ||||||||||||||||||||||||||||||||||||
| assay | 98% | ||||||||||||||||||||||||||||||||||||
| form | powder | ||||||||||||||||||||||||||||||||||||
| mp | 195 °C (dec.)(lit.) | ||||||||||||||||||||||||||||||||||||
| solubility | H2O: soluble5 mg/mL | ||||||||||||||||||||||||||||||||||||
storage temp. Sigma其他产品如下
|
2-8°C |
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文献和实验免疫检测仪OD490nm处测量各孔的吸光值。7.同时设置调零孔(培养基、MTT、二甲基亚砜),对照孔(细胞、相同浓度的药物溶解介质、培养液、MTT、二甲基亚砜)悬浮细胞:1)收集对数期细胞,调节细胞悬液浓度1×106/ml,按次序将①补足的1640(无血清)培养基40ul ;②加Actinomycin D(有毒性)10ul用培养液稀释 lg/ml,需预试寻找最佳稀释度,1:10-1:20);③需检测物10ul;④细胞悬液50ul(即5×104cell/孔),共100ul加入到96孔板(边缘孔用无菌水填充
的波动都不算奇怪。特别是新手,20%的波动也是常见的,所以很可能是技术原因引起的,特别是种板技术一定要过关。 3. 我做的是肿瘤细胞的MTT实验,这种细胞长的很快一开始我是用100 000/ML的浓度来接种的,结果细胞长的太满结果是没有梯度也没有线性关系.后来调整浓度,用过40 000~80 000/ML的浓度都做过MTT实验,结果发现做的结果比较好点的是60 000~70 000/ML的浓度组的.用40 000/M的浓度的组,由于细胞少,药物作用的梯度还是有,只是没有很好
。 扭矩控制式达到预先设定好的扭矩时自动断气源停止冲击。紧固螺栓或螺母时抵抗值上升造成运转切断气阀筒的安装方式。 型号 适用螺丝 扭力范围 空载转数 耗气量 气管接口 气管内径 全长 重量 mm N.m Kgf.cm
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