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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
N,N,N′,N′-Tetramethylethylenediamine BioReagent, for molecular biology, ≥99% (GC)
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
25ml
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| 01700-250G | 丙烯酰胺 |
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| 234907-100ML | 甲基丙烯酸二甲氨乙酯 |
| 155721-500G | 甲基丙烯酸 |
| 146072-100G | 甲叉双丙烯酰胺 |
| M8410-500ML | 矿物油 |
产品名称
包装
25, 50, 100 mL in glass bottle
Quantity
针对 50mL 丙烯酰胺凝胶溶液,建议用量为 35μL。
Application
This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.1,2,3
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.4 TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.5 Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.6
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.7 TEMED has been used in the HPLC of small organic molecules.8
性质
| Related Categories | Acrylamide, Gel Solutions and Reagents, 分子生物学, 核酸电泳, 自动测序
|
| grade | for molecular biology |
| product line | BioReagent |
| assay | ≥99% (GC) |
| expl. lim. | 9.08 % |
| refractive index | n |
| bp | 120-122 °C(lit.) |
| mp | −55 °C(lit.) |
| density | 0.775 g/mL at 20 °C(lit.) |
| foreign activity | DNase, RNase, protease, none detected |
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文献和实验://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=PMA&D7=0&D10=PMA&N1=S_ID&ST=RS&N25=0&F=PR 到底是那个啊 中介代理说是1000, 我还没给钱 chu465292278 chenfengyiguo wrote: 对了 6:sigma主页网上说List Price RMB是1000元
16*0.2ML 28,000 杭州大和热磁电子公司 PCR仪 杭州大和热磁电子有限公司 TC-25 16*0.2ML 30,800 杭州大和热磁电子公司 PCR仪 杭州大和热磁电子有限公司 TC-48/T/H 48*0.5ML 49,000 杭州大和热磁电子公司 PCR仪 杭州大和热磁电子有限公司 TC-88/T/H 88*0.2ML 88*0.2ML 52,500 杭州大和热磁电子公司 PCR仪 杭州大和热磁电子有限公司 TC-96/T/H 96*0.2ML 56,000 杭州大和热
聚丙烯酰胺凝胶电泳法(PAGE)分离血清蛋白质(核黄素-TEMED聚合系统)(图)
型电泳的薄片状凝胶时,太软很难操作。太硬则脆,也易折断。凝胶的透明度、粘着度是否合适也影响分离效果。凝胶的机械性能、弹性、透明度和粘着度取决于凝胶总浓度和Acr与Bis两者之比例。 凝胶密度或凝胶浓度,可以用二个字母T和C来定义。T代表丙烯酰胺和亚甲基双丙烯酰胺二个单体的总百分浓度。C代表总浓度T中交联剂Bis的百分浓度。Hjertern S.推导出如下这个式子,用以计算凝胶的用量。 这里 为丙烯酰胺的量(g) b为亚甲基双丙烯酰胺的量(g) m为缓冲液体积(ml) a/b的值
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