- ¥279.63
- sigma
- 进口
- T7024-25ml
- 2025年07月15日
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- 详细信息
- 技术资料
- 保存条件：
低温
- 英文名：
N,N,N′,N′-Tetramethylethylenediamine BioReagent, for molecular biology, ≥99% (GC)
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- 规格：
25ml
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产品名称
包装
25, 50, 100 mL in glass bottle
Quantity
针对 50mL 丙烯酰胺凝胶溶液，建议用量为 35μL。
Application
This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.1,2,3
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.4 TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.5 Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.6
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.7 TEMED has been used in the HPLC of small organic molecules.8
性质
| Related Categories | Acrylamide, Gel Solutions and Reagents, 分子生物学, 核酸电泳, 自动测序
|
| grade | for molecular biology |
| product line | BioReagent |
| assay | ≥99% (GC) |
| expl. lim. | 9.08 % |
| refractive index | n |
| bp | 120-122 °C(lit.) |
| mp | −55 °C(lit.) |
| density | 0.775 g/mL at 20 °C(lit.) |
| foreign activity | DNase, RNase, protease, none detected |
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