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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 应用范围:
WB
- 抗体英文名:
HIS Tag Antibody
- 抗体名:
HIS Tag Antibody
- 规格:
400ul
Mouse Monoclonal Antibody (Mab)
Application
WB
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1Clone
Names6AT18
| Target/Specificity Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody. |
|
| Dilution WB~~1:1,000 |
|
| Format Monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|
| Precautions HIS Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| 1 | AM1010a | HIS Tag Antibody |
| 3 | AP1012a | HA Tag Antibody |
| 4 | AM1008a | HA Tag Antibody |
| 5 | AM1009a | GFP Tag Antibody |
| 6 | AM1007a | Myc Tag Antibody |
| 7 | AM1011a | GST Tag Antibody |
| 10 | AM1011b | GST Antibody |
| 8 | AM1009b | GFP Tag Antibody (Ascites |
| 9 | AP1013a | FLAG tag antibody (DYKDDDDK |
| 12 | AP1017a | Protein-C Tag Antibody |
| 13 | AP1014a | V5 Tag Antibody |
| 14 | AP1016a | VSV-g Tag Antibody |
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文献和实验融合表达蛋白等。比如在免疫荧光IF的实验里,同时配合EarthOx的Dylight 549荧光二抗,使用该抗体可以准确的定位出Flag融合表达蛋白在细胞内的位置(如图红色显示部分)。另外,做融合表达分析前,通过免疫共沉淀技术获得Flag融合表达蛋白也是非常重要的一步,因此选择一个合适的、可用于免疫共沉淀(Immunoprecipitation,IP)Flag标签抗体也是很必要的。EarthOx的Anti-Flag Tag Monoclonal Antibody(货号#E022060)不仅能应用
Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
Antibody均能与GFP、YFP、EYFP、CFP等突变体有交叉反应,可以用于GFP、YFP、EYFP、CFP等荧光蛋白的表达定量、定位分析和检测。EarthOx该GFP标签抗体(货号#E022030)的另一个优势是,可用于的试验类型很多,除了基本的WB、ELISA试验外,还可以用于免疫细胞化学、免疫荧光(ICC/IF)、免疫共沉淀(IP)等。该抗体的效价很高,EarthOx的推荐WB起始稀释比率为1:1000,实际应用于中还能有更优异的表现。因此,EarthOx的Anti-GFP Tag
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