Cell Signaling CST 一抗 2978S Cy

【求助】细胞周期阻滞在G2/M期

while dephosphorylation by the phosphatase cdc25 activates the mitotic kinase. Proteins that localize to sites of DNA damage in G2 initiate a signaling cascade that regulates wee1 and cdc25 activity, therefore controlling mitotic entry via cdc2-cyclin B. Delay

Raf/MEK/MAPK Signaling Stimulates the Nuclear Translocation and Transactivating Activity of FOXM1

at late-G1 and S phases. Nuclear translocation occurs just before progression into the G2/M phase of the cell cycle and requires activity of the Raf/MEK/MAPK signaling pathway. Using pharmacological modulators, we demonstrated that activity of the Raf/MEK

湿兄，我有块蜡肉，怎么才能毕业？

检测系统，所以我们需要去除组织中内源性过氧化物酶的活性。只需在 3% H2O2 中浸泡 10 min 即可。此时的切片，便形似一张湿转后的 western blot 膜——方方的载体上分布着我们的蛋白。为了减少二抗与组织的非特异结合，我们需使用二抗来源的血清进行封闭。由于 CST 的绝大多数一抗为鼠抗（mouse）和兔抗（rabbit），对应的二抗来源于羊（goat），所以我们使用 5% 正常羊血清（溶剂为 TBST），将其滴加到组织上，室温封闭 1 h 即可。我们也可以使用 CST 新推出的不含