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文献和实验Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
Cy3 Cy5 mRNA (1 g/l ) x l y l (2 m g of each if mRNA, 50-100m g if total RNA
to differentiate into effector and memory Th cells with a wide variety of functional capabilities. The differentiation status of human resting Th cells can be distinguished by the cell surface expression of distinct isoforms of the molecule CD45 (1 ,2 ). Naive Th
Human CD4+CD25highCD127low/neg Regulatory T Cells
25, CD39/CD73, CD62L, CD45RO, CD127, glucocorticoid-induced tumor necrosis factor receptor (GITR), CTLA-4, and the forkhead/winged helix transcription factor (FOXP3), has been used to characterize Tregs. Tregs suppress T effector responses mainly
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