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文献和实验Quality-Control Testing of Mycoplasma Medium
, a complex growth medium is required for their culture. Although the exact requirements of most mycoplasma species are still incompletely defined, most can be grown in the laboratory in both liquid and solid agar media formulated to comprise as a minimum
The Cell Environment (including types of culture medium)
and epidermal growth factor. These media are usually HEPES bufferedInsect cells Sf-900 II SFM, SF Insect-Medium-2 (Prod. No. S3902) Specifically designed for use with Sf9 insect cells 1.Basic Constituents of media Inorganic
Agrobacterium growth and transformation
on the Gene Pulser, holding them until the tone sounds. Push the time constant display and note the time which should be ~9 msec (optimum conditions are reported to be those that give time constant between 8-12msec). 6. Add 1 ml of growth medium directly
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