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文献和实验Culture of Mouse Olfactory Sensory Neurons
Phosphate‐buffered saline (PBS; see recipe ) with 0.1% Triton X‐100 (PBS+TX) Normal horse serum
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
: Dilute (10x stock, -20ºC) 1/5 in PBS 2.5 N HCl: Dilute concentrated HCl 1/22 (v/v) in H2 O Mouse anti-BrDU: DAKO cat#M0744, $205/mL Biotinylated horse anti-mouse IgG, Avidin, biotin-HRP, Horse serum: Vector, Vectastain ABC kit, #PK4002 $185 Color
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