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文献和实验Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors
from murine fetal liver and human umbilical cord blood, which show physiological self-renewal responses to cytokine/hormone combinations, which in vivo are involved in stress hematopoiesis. We describe the expansion and sustained proliferation of multipotent
28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1 ), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human
Detection of FMR1 Trinucleotide Repeat Expansion Mutations Using Southern Blot and PCR Methodologies
basis for nearly all mutations (>99%) in the gene and leads to reduced or complete loss of FMRP (3 –8 ). The variable phenotype occurs related to variation in FMR1 expression mediated by the extent of CGG repeat expansion and a secondary epigenetic
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