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文献和实验Keratinocyte Culture in the Absence of Substrate Attachment
of loss of extracellular matrix interaction on crucial aspects of epithelial cell biology. Specifically, examining cells in the absence of substrate attachment, as described in this chapter, will allow the investigator to study the effect of growth factors
ELISA on Attachment-Dependent or Suspension Grown Cells
(2). The antigen (or antibody) is immobilized in the well of a 96-well plate. An enzyme-conjugated antibody (or antigen) is then added to the well to bind to a specific target. Any unbound antibody (antigen) is rinsed away. Substrate is added to react
Assays for RING Family Ubiquitin Ligases
through an enzymatic cascade of three discrete steps, which results in covalent attachment of ubiquitin to the substrate. The first two steps in this cascade involve the activating and conjugating enzymes, E1 and E2. The third and final step is performed by the E3
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