- ￥1 - 4998
- Stemcell
- PT-3298
- 中国/美国
- 2025年08月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1 EA
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Bisulfite sequencing of very small samples
Incubate the bead with 500 µL of 0.2 M NaOH for 15 minutes at room temperature, remove NaOH. Incubate the bead with 500 µL of 0.2 M NaOH for 15 minutes at 37 °C. (desulfonation step) Neutralize NaOH with 100 µL of 1M hydrochloric
Cloning of small RNAs with 5’ phosphate and 3’ OH ends
of small RNA (from 10 to 35 µg total RNA). Note, we also use Arabidopsis floral tissue, which has a high percentage of small RNAs (comment 1). Prepare the 15% 8M UREA gel. Pour a 15% UREA gel in the Biorad MiniProtean 2, using 0.75mm spacers
Bisulfite sequencing of small DNA/cell samples
of Proteinase K (20 mg/ml) and 5 µg of yeast t-RNA (use 1.5 ml Eppendorf tubes ) Incubate 1h at 37°C Add 50 µl of phenol and 50 µl of chloroform/isoamylalcohol (24:1) Mix the samples gently by inverting the tubes Centrifuge
技术资料暂无技术资料 索取技术资料
