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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Erythrina caffra Trypsin inhibitor DE-3
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Erythrina caffra Trypsin inhibitor DE-3品名简称:
Recombinant Erythrina caffra Trypsin inhibitor DE-3 protein货号:
CSB-EP362840EGO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P09943别名:
Trypsin inhibitor DE-3种属:
Erythrina caffra (Kaffir tree) (Coastal coral tree)蛋白长度:
Full Length来源:
E.coli分子量:
35.3 kDa表达区域:
1-172aa氨基酸序列:
VLLDGNGEVVQNGGTYYLLPQVWAQGGGVQLAKTGEETCPLTVVQSPNELSDGKPIRIESRLRSAFIPDDDKVRIGFAYAPKCAPSPWWTVVEDEQEGLSVKLSEDESTQFDYPFKFEQVSDQLHSYKLLYCEGKHEKCASIGINRDQKGYRRLVVTEDYPLTVVLKKDESS蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Inhibition of trypsin.功能2:
Inhibition of trypsin.文献:
The primary structure of the inhibitor of tissue plasminogen activator found in the seeds of Erythrina caffra.Joubert F.J., Dowdle E.B.D.Thromb. Haemost. 57:356-360(1987)研究领域:
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文献和实验胰蛋白酶抑制剂 trypsin inhibitor 由生物产生,为与胰蛋白酶结合具有抑制该酶作用的物质。最初在胰脏中发现,但多数发现于血浆、豆科植物的种子、鸟类的卵等。认为动物的胰蛋白酶抑制剂的作用是调节胰蛋白酶的活性,但植物中的这种物质,其存在的意义还不清楚。它和胰蛋白酶形成 1∶ 1复合体,覆盖着活性部位起抑制作用。一般其结合力相当强,特异性也高,但有的对胰糜蛋白酶等其它蛋白酶也起抑制作用。上述列举的是低分子蛋白质,但最近从放线菌中发现一种新类型的物质,称为白肽
蛋白质技术中常常要用到lysis buffer,各个实验室的lysis buffer的配方是不同的,开设个专题,希望大家详细谈谈自己使用的lysis buffer的配方,以及各个组成成分的作用,方便广大蛋白质战友。 先介绍一下我们用的: PBS 缓冲液,不用多说; TRITON X-100 Triton X-100中文名为曲拉通X-100,分子式为t-Oct-C6H4-(OCH2CH2)xOH, x=9-10,平均分子量为647。 常用的非离子性去垢剂,主要
inhibitor is ineffective. McKeehan and Ham report markedly improved viability and multiplication potential to single cells in low serum medium when harvesting with crystalline trypsin at reduced temperatures, i.e., at 4°C. Cell
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