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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human U6 snRNA-associated Sm-like protein LSm4 (LSM4)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥10872.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2514.0 |
| 规格: | 20μg | 产品价格: | ¥1344.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
LSM4Uniprot No.:
Q9Y4Z0别名:
Glycine-rich protein ;GRP种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
31.3 kDa表达区域:
1-139aa氨基酸序列:
MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKGRGRGGLQQQKQQKGRGMGGAGRGVFGGRGRGGIPGTGRGQPEKKPGRQAGKQ蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Binds specifically to the 3'-terminal U-tract of U6 snRNA.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Epigenetics and Nuclear SignalingReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014)Function:
Binds specifically to the 3'-terminal U-tract of U6 snRNA.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验师从施一公,发表 6 篇Science/3篇 Cell,她被誉为「世界最具潜力女科学家」| 论文盘点
的双链被 Yhc1、Luc7 和 Sm 环识别。 在 B 复合体中,U1 小核糖核蛋白被解离,5’-外显子- 5’sS 序列移位到 U6snRNA 附近,3 个 B-特异性蛋白可能定位前体信使 RNA。 在这两个复合物中,U6 snRNA 锚定在 U5 snRNA 的环 I 上,分支点序列和 U2 snRNA 之间的双链被 SF3B 复合物识别。Pre-B 复合体的结构阐明填补了剪接体对 Pre-mRNA 剪接的机制理解中的重要知识空白,揭示了酵母剪接体的组装和激活机制。 酿酒酵母 Pre
施一公团队又出新作!进一步解析人类 U2 snRNP 组装过程,提供癌症突变机制新见解
导读 pre-mRNA 的剪接是由一种被称为剪接体的大而动态的 RNA-蛋白质复合物执行的。剪接体由 5 个小的核糖核蛋白颗粒(snRNPs,包括 U1、U2、U4、U5 和 U6)和非 snRNP 因子组装而成。每个 snRNP 由一个单独的小核 RNA (snRNA),7 个常见的 Sm 蛋白和一些特定的蛋白质因子构建。在这 5 个 snRNP 中,U2 snRNP 在内含子的识别和前体折叠的组装过程中起着重要作用。 人类的 U2 snRNP 尤其复杂,它的组装是一个多步骤的过程
.7. Wang, X. et al. 2011. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Sci Rep1: 102 (DOI:10.1038/srep00102).8. Wang, X. and Zhang, S. 2011. Production of a bioengineered G-protein coupled receptor of human
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