MYT1|MYT1蛋白|MYT1人蛋白|MYT1 Prote

Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling

in an inactive form (pre-MPF) during the G2 arrest owing to the phosphorylation of Cdc2 by the Myt1 protein kinase. The dephosphorylation necessary to activate pre-MPF and induce progression of the oocyte into meiosis is catalyzed by the phosphatase Cdc25C.

【原创】Protein A Sepharose 之进化篇。。。

洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下

Purification of Human Complement Protein C5

Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. Whereas purification of its paralogues C3 and C4 from plasma is relatively