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- 详细信息
- 文献和实验
- 技术资料
- 样本:
viral supernatant and purified virus
- 检测方法:
enzyme immunoassay
- 检测范围:
1 ng/mL HIV p24
- 库存:
大量
- 供应商:
深圳欣博盛生物科技有限公司
- 规格:
5 x 96 assays
品牌: cell biolabs
产品货号: VPK-107
规格: 96 assays
产品信息:
Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present.
Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.
慢病毒快速滴度试剂盒(慢病毒相关p24 ELISA)的检测原理:

ViraBind™ 慢病毒试剂成功地将游离p24与慢病毒相关p24分离结果如下:

关于Cell Biolabs
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文献和实验2. Verma, I. M., and N. Somia (1997) Nature 389, 239-242
3. Kahl C. A., Marsh J., Fyffe J., Sanders D. A., and K. Cornetta (2004) J Virol. 78:1421-30.
4. White S. M., Renda M., Nam N. Y., Klimatcheva E., Zhu Y., Fisk J., Halterman M., Rimel B. J., Federoff H., Pandya S., Rosenblatt J. D., and V. Planelles (1999) J Virol. 73:2832-40.
5. Kafri T., van Praag H., Ouyang L., Gage F. H., and I. M. Verma (1999) J Virol. 73:576-84.
Gene Delivery by Lentivirus Vectors: An Overview
-titer concentrated retrovirus vectors (>109 infectious units [IU]/mL) can be generated by several retrovirus-vector stable producer lines. The ability to pseudotype retrovirus vectors with a variety of envelope proteins, including the vesicular
慢病毒转染肝细胞方法Lentivirus Transduction of Hematopoietic Cells
Lentivirus Transduction of Hematopoietic Cells Ming-Jie Li and John J. Rossi Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010
以更少量的样本完成对更多生物标志物的分析。由卓越的免疫试剂供应商开发,用于 Luminex® 平台,ProcartaPlex 使您可以在 25-50μL 的样本(血浆、血清、细胞培养上清液以及其他体液)。实现在 25-50μL 的样本中同时对多达 50 种因子进行检测与定量。该技术在多通道「类 ELISA」分析中,为每种靶标使用了经不同染色处理的捕获微球。相比于传统的三明治 ELISA 而言,ProcartaPlex 免疫分析可以在使用样本量少而时间相同的情况下,分析多达 50 倍
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