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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid Hsp70. The target is not present in any other Hsp70 proteins.
- 保存条件:
4°C
- 克隆性:
Polyclonal
- 标记物:
Alkaline Phosphatase
- 适应物种:
The antibody is specific for salmonid Hsp70 (tested on salmon and brook trout). It does NOT cross react with Hsc70. It does not detect Hsp70 in other species.
- 宿主:
Rabbit
- 应用范围:
WB
- 抗体英文名:
Hsp70 (Fish) Antibody: Alkaline Phosphatase
- 规格:
100μl
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文献和实验Alkaline Phosphatase (EC 3.1.3.1)
bacteria, fungi, invertebrates, fish, and mammals (being located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most commonly used alkaline phosphatases (AP
Conjugation of Antibodies to Alkaline Phosphatase
Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedure using the homobifunctional reagent glutaraldehyde, which reacts with amino groups in the two proteins. The procedure is simple to perform and requires minimal
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
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