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- 英文名:
Pierce™ Mouse Embryonic Fibroblast Isolation Kit
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嵘崴达
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1 个试剂盒
Pierce™ 小鼠胚胎成纤维细胞分离试剂盒
货号: 88279
The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.
Features of the Mouse Embryonic Fibroblast Isolation Kit:
• Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
• Functional – cultured fibroblasts express the appropriate biochemical markers
Includes:
• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation
The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with the Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.
Applications:
• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies
| Pierce | 88279 | Pierce Mouse Embryonic Fibroblast Isolation Kit | 1L |
| Pierce | 88280 | Pierce Primary Neuron Isolation Kit | DAFA Kit |
| Pierce | 88281 | Pierce Primary Cardiomyocyte Isolation Kit | 1L Kit |
| Pierce | 88282 | Pierce LAL Chromogenic Endotoxin Quantitation Kit | Kit |
| Pierce | 88283 | Neuronal Culture Medium | 500 mL |
| Pierce | 88284 | Hanks’ Balanced Salt Solution (HBSS, without Ca2+, Mg2+) | 500 mL |
| Pierce | 88285 | Neuronal Isolation Enzyme (with papain), lyophilized | 1 VIAL |
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文献和实验相关专题 Thermo Scientific Pierce近日推出了HiPPR去垢剂去除离心柱/离心板,可更快更彻底地从细胞裂解液及其他生物样品中去除去垢剂。这种即用型的产品适用于低浓度的蛋白及多肽样品。HiPPR树脂结合并去除去垢剂,同时很好地回收蛋白及多肽,便于下游的质谱分析。 大家都知道,去垢剂和表面活性剂是制备蛋白和多肽样品时常常使用的成分。然而,它们也会干扰下游的ELISA、等电聚焦和质谱分析。请
小鼠胚胎成纤维细胞的富集1、给13-14天的孕鼠注射大约0.5ml阿佛丁。当鼠麻醉后,实施断颈法处死小鼠。2、用70%乙醇擦拭腹部,把皮肤向后拉,暴露出腹膜。用消毒过的工具,剪开腹壁以暴露出子宫角。将子宫角移到10cm的皿里。用10ml不含钙镁离子的PBS洗三遍。3、用剪刀剪开每一测的胚囊,并将胚胎移到培养皿中。4、用两副钟表镊子将胎盘和膜与胚胎分离开,分离后切除内脏(所有深色的东西)。将胚胎转移到(有盖)培养皿中,用10ml不含钙镁离子的PBS洗三遍。5、用带有弯钩的眼科剪将组织剪碎,当你剪
近年来,ZFN、TALEN、CRISPR/Cas9等基因组编辑技术可谓是层出不穷,尤其是CRISPR/Cas9,一出现便在全球范围内掀起一股热潮。同时,各大公司也开发出一系列相应的产品,其中一种至关重要的就是基因敲除阳性克隆筛选的试剂。 目前,市面上只有Genloci的Cruiser™、Transgenomic的SURVEYOR和NEB的T7EI这三种产品和测序这种方法,被用于基因敲除阳性克隆的筛选。然而,SURVEYOR是进口产品,价格贵,货期长;T7EI可以识别多种DNA结构,如十字
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