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- BD biosciences
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- 美国
- 2025年07月12日
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上海恪敏生物科技有限公司
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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
0.05% Tween-20 for 2 minutes, each time. 4) Proceed immediately to Signal Development, below. Signal Development 1) Dilute the 20X DAB Chromogen supplied with the kit in a sterile microcentrifuge tube just prior to use
from Vector DAB substrate kit (Catalog No. SK-4100), four drops DAB, two drops H2O2 , and two drops of Nickel from kit, in 15ml tube (wrap the tube in foil to protect the substrate from light) . Incubate in the dark for 20-30 min., stop color reaction
RAT/MOUSE GROWTH HORMONE ELISA KIT
peroxidase to the immobilized biotinylated antibodies, 6) wash away of free enzyme conjugates, and 7) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate
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