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- 英文名:
Collagenase from Clostridium histolyticum Type I
- 库存:
5
- 供应商:
研卉生物
- CAS号:
9001-12-1
- 规格:
100mg
胶原蛋白酶 来源于溶组织梭菌
Collagenase from Clostridium histolyticum
Type I, for general use, Vetec™ reagent grade, powder, ≥125 CDU/mg solid
MSDS
别名: 梭菌肽酶 A
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Quality
也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type I has the original balance of collagenase, caseinase, clostripain, and tryptic activities, and is suggested for epithelial, liver, lung and adrenal primary cell isolations.
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文献和实验although some are more promiscuous. Three types of DNA methyltransferases have been found as part of Type II R-M systems forming either C5-methylcytosine, N4-methylcytosine or N6-methyladenine. 酶 类型 识别序列 ApaI Type II restriction enzyme 5'GGGCC^C 3' BamHI Type II restriction
: (1)引物的长度一般为15-30 bp,常用的是18-27 bp,但不应大于38,因为过长会导致其延伸温度大于74℃,不适于 Taq DNA 聚合酶进行反应。 (2)引物序列在模板内应当没有相似性较高,尤其是3’端相似性较高的序列,否则容易导致错配。引物3’端出现3 个以上的连续碱基,如GGG 或CCC,也会使错误引发机率增加。 (3)引物3’端的末位碱基对Taq 酶的DNA 合成效率有较大的影响。不同的末位碱基在错配位置导致不同的扩增
,而 AA 大鼠会出现免疫功能紊乱,且缺乏慢性病理过程,有一定的局限性。 2. 胶原诱导的关节炎(CIA) CIA 是 Trentham[5] 等 1977 建立的实验性关节炎动物模型,多种动物的 Ⅱ 型胶原均可引起关节炎,但多用啮齿类和灵长类。CIA 作为一种内源性自身抗原介导自身免疫性疾病,是目前研究 RA 的金标准模型[6]。 造模机制:胶原蛋白(collagen)是细胞外间质成分,分为 Ⅰ 型、Ⅱ 型和 Ⅲ 型,其中 Ⅱ 型胶原大量存在于关节软骨中。当用异源性 Ⅱ 型胶原(如牛 CⅡ 或鸡 CⅡ
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