万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
25 UG
- 供应商:
南京赛泓瑞
- 规格:
100 μl
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验antibody at 5 µg/ml for 45 min at 4 °C. Mouse anti-human NFB p65 monoclonal antibody sc-8008 (Santa Cruz, CA, USA) recognizes epitopes mapping the C amino-acid terminus of human origin NFB p65. After two washes in PBS–FCS, nuclei were incubated for 45 min
-hot polyclonal, or 1:30 dilution of tissue culture supernatant for a good monoclonal. Do the incubation in a sealameal bag for 1 hr at room temp on a rocker. Can go overnight in the fridge. Remember: it's critical to do a control with no 1° antibody
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) could be detected in the bound fraction after precipitation with conventional mono- and polyclonal antibodies (Figure 1) (Rothbauer et al., 2008). The lack of unspecific binding or antibody fragments is one major advantage of the GFP-Trap, because unspecific protein
技术资料暂无技术资料 索取技术资料





