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南京赛泓瑞
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
are performed by inducing in vitro and in vivo apoptosis and then visualized the phenomenon with anti-DNA mAb. In vitro experiments are performed by treating human lymphocytes with 2-deoxyribose and rat thymocytes with dexamethasone 21-phosphate. In vivo
,表现出Th1、Th2和TFH等具有传统功能的细胞亚群。导致Foxp3下调的关键因素包括高水平的炎症因子内环境,如可诱导效应性T细胞产生的细胞因子:IL-6和IFN-gamma。Tregs 细胞中Foxp3的表达分析CD4+CD25+ Treg 细胞中Foxp3 +细胞群表明与Foxp3+Treg完全重合。使用Foxp3鉴定Treg用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上
免疫组化技术-Immunohistochemical Protocols
in the same section. You can use the above protocols and do the two stainings simultaneously. (A brief example: first antibody (raised in rabbit); anti-rabbit-IgG-FITC; second primary antibody (raised in mouse); anti-mouse-IgG-CY3. Result: one antigen shows
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