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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温
- 英文名:
SILICA GEL 60 F254, PLC PLATES
- 供应商:
西宝生物
- 库存:
12SHEET
- 规格:
100克/500克
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文献和实验for downstream digests oheat 5 minutes at 60 degrees ospin 2 min @ full speed in microcentrifuge oremove DNA-containing supernatant to clean tube Notes & Misc: •Conveinient PCR clean up before digestion: 100μl PCR reaction cleaned up with 50μl silica
resuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer for downstream digests heat 5 minutes at 60 degrees spin 2 min @ full speed in microcentrifuge remove DNA -containing supernatant to clean tube Notes & Misc
Preparation of a DNA Sequencing Gel
of the plate with detergent and water using crub brush and wet paper towel. c. Rinse the plate well with tap water, running your hands over the gel to detect any residual polyacrylamide. d. Set the plates on the drying rack to drain. e. Dry
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