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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
西宝生物
- 保存条件：
室温
- 库存：
40RXN
- 英文名：
PSTBLUE-1 PERFECTLY BLUNT(R) CLONING KIT
- 规格：
96T/48T
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文献和实验3’-A overhang to each end of the PCR product. Blunt cloning vectors and directional TOPO® cloning technologies are designed to clone PCR products produced by proofreading polymerases. Successful cloning depends upon using the correct polymerase with your cloning
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
Cloning PCR products using TA vectors
which can be used for this, including the pBluescript derivative pCR-Script [TM] SK(+) cloning kit from Stratagene, pCR [TM] II supplied with the TA Cloning [TM] kit from Invitrogen, pT7Blue T-Vector kit from Novagen, pGEM[R]-T from Promega, and the SureClone [TM
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