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文献和实验Troubleshooting for PCR and multiplex PCR
at the 3' end. If size is not a concern, add a few bases at either the 3' or the 5' end of that primer. 6. I have a number of primer pairs I would like to use together. Can I run a multiplex PCR with them?. How? Very likely, yes. Try amplify all loci
Multiplex PCR Primers Richard Souvenir; Jeremy Buhler; Gary Stormo; Weixiong Zhang pp. 245 - 268 13. Primer Design for Multiplexed Genotyping Lars Kaderali pp. 269 - 286 14. MultiPLX: Automatic Grouping and Evaluation of PCR Primers Lauris
Absolute value of primer concentration in multiplex PCR . The amount of DNA primer(s) available during the PCR reaction influences the results. Primer concentration taken in a common PCR reaction (for example
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