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文献和实验Negative Stain Electron Microscopy of Microtubules
in coldroom for several hours till fully dissolved. Filter through a 0.22 µm filter that has be prerinsed well with ddH2 O. Filtered stain stored at 4¡C in a foil-wrapped tube can be used for >1 year.) Filter strips (prepared by cutting Whatman #1 filter
Vybrant® DyeCycle Violet Stain
Stability Vybrant® DyeCycle™ Violet stain 200 μL 5 mM solution in deionized water 2-6?C
Negative Stain Electron Microscopy of Microtubules
Negative Stain Electron Microscopy of Microtubules Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains
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