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文献和实验Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
as otherwise all available Mg2+ is chelated out of solution and enzyme reactivity is adversely affected. Any increase in dNTPs over 200uM means [Mg2+] should be re-optimised.AVOID USING EDTA-CONTAINING BUFFERS AS EDTA CHELATES Mg2+Low primer, target, Taq, and nucleotide
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984
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