Taq DNA Polymerase 10 x React

The Polymerase Chain Reaction (PCR)

• Template DNA　　• Reaction buffer (Tris, ammonium ions(and/or potassium ions), magnesium ions,bovine serum albumin)　　• Nucleotides (dNTPs)　　• Primers　　• DNA polymerase (usually Taq) PCR In Detail　　• Denature, anneal, extend and repeat the cycle 30 to 35

Taq Polymerase Purification

This is a protocol from Engelke et.al.(1990)which was modified by Dr.Baron.The enzyme has been used for RT-PCR,genotyping and cloning.Solutions1000X IPTG0.5M IPTG 1.19g IPTGup to 10 ml wih sterile Qfilter and store at -20℃Buffer A50 mM Tris 7.9 25

Taq酶PCR实验方法介绍

for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended