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- 2026年01月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Propylene Glycol Standard
- 供应商：
富士胶片和光
- 规格：
100mg
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文献和实验Purification of dnEBNA-1/Soft from E. coli BL21 LysS
and save 50μl as Wash 1. Wash with 15ml TE+0.5M NaCl,collect in 5ml fractions.Save 50μl of each as Wash 2.1,Wash 2.2,Wash 2.3. Elute dnEBNA-1/Soft from column with 5-10ml TE+0.7M NaCl+30% propylene glycol,collect in 1ml fractions.Add 5μl of 0.02M DTT
Temperature-Induced Phase Partitioning for Protein Purification
Ethylene oxide (EO)-propylene oxide (PO) random copolymers are watersoluble and separate into two phases above a lower critical solution temperature (LCST), i.e., the cloud point, a polymer-rich phase, and a water phase depleted of polymer
that have not been previously been cleared in BB:BA, it makes them too fragile. Wax. Standard protocol. 1) take samples in 100% methanol, remove methanol and replace with 100% ethanol, leave 5' 2) exchange ethanol with fresh 100% ethanol, leave 5'
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