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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Propylene Glycol Alginate, 30 - 70mPa・s
- 供应商:
富士胶片和光
- 规格:
500g
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文献和实验胚胎和成年斑马鱼眼情的组织学准备Histological Preparation of Embryonic and Adult Zebrafish Eyes
2-Dodecenylsuccinic acid anhydride (DDSA) 60 mL DMP-30 1.1 mL Solution must be mixed thoroughly. Store at -20°C.
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
and save 50μl as Wash 1. Wash with 15ml TE+0.5M NaCl,collect in 5ml fractions.Save 50μl of each as Wash 2.1,Wash 2.2,Wash 2.3. Elute dnEBNA-1/Soft from column with 5-10ml TE+0.7M NaCl+30% propylene glycol,collect in 1ml fractions.Add 5μl of 0.02M DTT
all the embryos in the same vial without confusing them. 17. Rinse the blocks in dH20 and transfer to a vial. I used a scintillation vial. 18. Dehydration: 6' 30% EtOH 10' 50% EtOH 10' 70% EtOH * .. 70% EtOH
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