核酸酶P1 Nuclease P1

核酸酶P1分解RNA和变性DNA的3'-5'磷酸二酯键，同时也分解单核苷酸和寡核苷酸的 3'-磷酸单酯键。在氯化钠浓度在400mmol/L或者更高（pH6.0），不会影响双链DNA。

Usage example
A. Sample : 200uL of RNA or heat-denatured DNA at a density of 2mg/mL.
B. Reaction Buffer : 200uL of 0.01 mol/L barbital(veronal) acetate buffer, pH5.3*.
C. Enzyme : 100uL of 100 units/mL of Nucelase P1 solution with distilled water.
D. Stop Solution : 500uL of cold uranyl acetate buffer(0.25% of uranyl acetate and 2.5% perchloric acid)

*barbital(veronal) acetate buffer, pH5.3 : Prepare 0.01mol/l Barbital Sodium Salt in water and adjust it to pH 5.3 with acetic acid.

1. Mix each 200uL of A, B and C.
2. Incubate it at 50 degree C for 1 hour.
3. The reaction can be stopped by adding 500uL of D.

Reference
Masao Fujimoto, Akira Kuninaka & Hiroshi Yoshino(1973)Purification of a Nuclease from Penicillium citrinum, Agr. Biol. Chem.,38(4), 777-783.