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文献和实验Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
, the reduced thiols are first blocked with N -ethyl-maleimide, then the oxidized thiols reduced with dithiothreitol and labelled with 5-iodoacetamidofluorescein. The method is illustrated by treating Jurkat T-lymphoma cells with hydrogen peroxide and monitoring
Preparation of Carboxypeptidase Y and Its Properties
through polyacrylamide gel electrophoresis. Carboxypeptidase Y possesses the activity of esterase and peptase that can be assayed using N-acetyl-L-tyrosine ethyl ester (ATEE) and benzyloxycarbonyl-L-phenylalanyl-L-leucine (CBZ-Phe-Leu) as substrates respectively
by Zbigniew Darzynkiewicz, Cancer Research Institute, New York Medical College. HL-60 cells（急粒，p53缺陷）被喜树碱诱导3小时。DNA段端被荧光标记的脱氧核苷酸末端转移酶标记。 Detection of apoptosis in SK-N-MC neuroblastoma cells. Following a six-hour exposure to hydrogen peroxide, cells
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