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- 2025年07月07日
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- 保存条件：
Endoproteinase Glu C, Sequencing grade
- 库存：
大量
- 供应商：
FUJIFILM Wako
- 规格：
50ug
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文献和实验Targeted Sequencing Using Affymetrix CustomSeq Arrays
(unit 14.4 ) Human genomic DNA (positive control; Clontech cat. no. 6550‐1; store at 4°C) Molecular biology–grade water
Cycle Sequencing Protocol Using 33P-Radiolabeled Terminators
tends to produce high background and ghost bands.) 1.Prepare the 4 termination mixes by combining 2.0ul of dGTP-termination-master-mix and 0.5ul of labeled ddNTP for each sequence. 2.For each set of sequencing reactions, label four PCR grade microfuge
either by ultraviolet (UV)-irradiation or by combining 2-iminothiolane and UV treatment. The generated RNA-protein crosslinks are digested with ribonuclease T 1 and endoprotease (Lys-C or Glu-C) and purified by size-exclusion chromatography and reverse phase-high
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