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文献和实验Bicinchoninic Acid (BCA) Protein Assay (Smith)
)has suggested that the BCA assay will replace the Lowry because it requires a single step,and the color reagent is stable under alkaline conditions. Both a standard assay for concentrated proteins and a micro assay for dilute protein solutions are described
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODS
) Micro-BCA Protein Assay (pdf) 0.5-20 µg/ml Compatible with detergents solubilized proteins. Proteins on affinity supports. Chaotropic agents, sugars, DNA, protease inhibitors 1ml reagent + 1ml sample
oligo (IntegratedDNA Technology, Coralville, IA, USA) is recommended. 3. PCR reagents: Herculase II fusion polymerase (Agilent, SantaClara, CA, USA). Other high-fi delity polymerases, such asKapa HiFi (Kapa Biosystems, Wilmington, MA, USA) can alsobe
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