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文献和实验Prion Protein as Copper-Binding Protein at the Synapse
(1 ). The glial fibrillary acidic protein was one of the first possible binding partners to be described (2 ) followed by Bcl-2 (3 ,4 ), molecular chaperones (5) , amyloid precursor-like protein 1 (6 ), the 37-kDa laminin receptor (7 ) and a 66-kDa membrane
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
BioID: A Screen for Protein‐Protein Interactions
, the vast majority of the biotin signal, detected with streptavidin–Alexa Fluor 488 (green), co‐localizes with the fusion protein. DNA is detected with Hoechst dye (blue). Scale bar is 15 µm. (B ) Following SDS‐PAGE separation, the protein constituencies
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