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文献和实验Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
binding peptides. We present here a high content screening for MHC class II (HLA-DR) binding to a peptide library in a chip-format. Binding of soluble MHC class II molecules to individual peptides can be visualized using an anti-DR directed monoclonal
Class II MHC Antigen Processing in Phagosomes
(MHC II) molecules and class I MHC (MHC I) molecules to stimulate CD4+ and CD8+ T cells, respectively. To examine the role of phagosomes in processing of antigens for presentation by MHC II molecules to CD4+ T cells, phagosomes have been biochemically
In Vitro Digestion with Proteases Producing MHC Class II Ligands
digestion assays of protein with recombinant proteases or purified endosomes/lysosomes, T cell stimulation) to monitor the production of MHC class II ligands.
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