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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
proliferation. What You Need Materials 1X sterile PBS Anti-mouse CD3e, 145-2C11: Functional Grade, Cat. No. 16-0031 , or Purified, Cat. No. 14-0031 Supplemented RPMI-1640 click
in vitro proliferation of mouse splenic T-cells and human peripheral T cells stimulated via CD3. All steps where optimization by the user can be achieved for meaningful results have been highlighted. Briefly, these include titration of the cell
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