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- 详细信息
- 文献和实验
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- 克隆性：
多克隆
- 规格：
100 ug
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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Western Blotting with Horseradish Pe
ELECTROPHORESIS Guidelines for choosing the percent gel to be used for certain molecular weight proteins (based on 37:1 acrylamide: bis acrylamide ratio) 4-5% gels: > 250 kDa 7.5% gels: 250-120 kDa 10% gels: 120-40 kDa 13% gels: 40-15 kDa 15% gels: Gel
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