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- 文献和实验
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- 保存条件：
RT
- 保质期：
10年
- 英文名：
北诺
- 库存：
1000
- 供应商：
北诺
- 规格：
1个/包
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文献和实验Blood collection and administration of fluids and drugs (mouse)
a successful peri-orbital bleeding technique. Pressure should be applied after blood collection to prevent hematomas. Anesthesia is required for all peri-orbital bleeding procedures. Axillary bleed : Equipment: scalpel blade 3 to 5 cc syringe Blood
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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