- ￥3480
- Thermo Scientific
- USA
- 26617(SM0672)
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2年
- 英文名：
thermo
- 库存：
1000
- 供应商：
北诺生命科学
- 规格：
10 X 250 UL
公司产品线包括：FastDigest & 常规限制性内切酶、修饰酶、PCR & RT-PCR产品、核酸和蛋白质Markers、分子生物学试剂盒（分子克隆、核酸纯化、标记、检测）、转染试剂、核苷酸等。
Unstained Protein MW Marker, 2 x 1 mL. 26610(SM0431) Pierce
Prestained Protein MW Marker, 500 uL. 26612(SM0441) Pierce
PageRuler Unstained Protein Ladder, 2 x 250 uL. 26614(SM0661) Pierce
PageRuler Prestained Protein Ladder, 2 x 250 uL. 26616(SM0671) Pierce
PageRuler Prestained Protein Ladder, 10 x 250 uL. 26617(SM0672) Pierce
PageRuler Plus Prestained Protein Ladder, 2 x 250 uL. 26619(SM1811) Pierce
PageRuler Plus Prestained Protein Ladder, 10 x 250 uL. 26620(SM1812) Pierce
Spectra Multicolor Broad Range Protein Ladder, 10 x 250 uL. 26623(SM1842) Pierce
Spectra Multicolor High Range Protein Ladder, 2 x 250 uL. 26625(SM1851) Pierce
Spectra Multicolor Low Range Protein Ladder, 250 uL. 26628(SM1861) Pierce
PageRuler Broad Range Unstained Protein Ladder, 26630(SM1881) Pierce
PageRuler Low Range Unstained Protein Ladder, 2 x 250 uL. 26632(SM1891) Pierce
Spectra Multicolor Broad Range Protein Ladder, 2 x 250 uL. 26634(SM1841) Pierce
10X Buffer Cfr9I B02 Fermentas
10X Buffer Cfr10I B04 Fermentas
10X Buffer EcoRI B12 Fermentas
10X Buffer Bsp143I B13 Fermentas
Bovine Serum Albumin B14 Fermentas
10X Taq Buffer with KCl and 15 mM MgCl2 B16 Fermentas
Dilution Buffer for Restriction Enzymes B19 Fermentas
10X Buffer Eco52I B22 Fermentas
10X Buffer SduI, Ppu21I B23 Fermentas
10X Buffer SdaI B24 Fermentas
10X Buffer Eam1105I B25 Fermentas
10X Buffer Ecl136II, PacI, SacI B26 Fermentas
10X Buffer AarI, AjiI, Bpu10I, ScaI, PasI B27 Fermentas
10X Buffer TaqI B28 Fermentas
10X Buffer KpnI B29 Fermentas
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文献和实验C30min（3ul？多加点没关系） 7)蛋白酶K37C30min（3ul？多加点没关系）再浓缩浓缩 8)6xloading buffer 混合根据小片段液量自己选择（6－10ul）0.5xTBE里跑电泳 注意：DNA别降解了，混匀时tip头口剪宽一些，组织要多一些。 另：你们问我如何避免样品溢出，好像溢出不多啊，一丝一缕而已，至少我的ladder跑得出来，就把胶放在电泳槽里后再加TBE吧，不至于全流出来吧，还有就是用6X的loading buffer,可能可以增加比重，这也是书上的做法。要不把胶
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
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