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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4度,-20
- 保质期:
2年
- 英文名:
thermo
- 库存:
1000
- 供应商:
北诺生命科学
- 规格:
100 ul
货号 产品名称 规格 2013 目录价(¥)
K0331 Luminaris Color Probe High ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0332 Luminaris Color Probe High ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0333 Luminaris Color Probe High ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0334 Luminaris Color Probe High ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0341 Luminaris Color Probe Low ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0342 Luminaris Color Probe Low ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0343 Luminaris Color Probe Low ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0344 Luminaris Color Probe Low ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0351 Luminaris Color Probe qPCR Master Mix 250 x 20 µL rxns 1,355
K0352 Luminaris Color Probe qPCR Master Mix 500 x 20 µL rxns 2,510
K0353 Luminaris Color Probe qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0354 Luminaris Color Probe qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0361 Luminaris Color HiGreen High ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0362 Luminaris Color HiGreen High ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0363 Luminaris Color HiGreen High ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0364 Luminaris Color HiGreen High ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0371 Luminaris Color HiGreen Low ROX qPCR Master Mix 250 x 20 µL rxns 1,355
K0372 Luminaris Color HiGreen Low ROX qPCR Master Mix 500 x 20 µL rxns 2,510
K0373 Luminaris Color HiGreen Low ROX qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0374 Luminaris Color HiGreen Low ROX qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0381 Luminaris Color HiGreen Fluorescein qPCR Master Mix 250 x 20 µL rxns 1,355
K0382 Luminaris Color HiGreen Fluorescein qPCR Master Mix 500 x 20 µL rxns 2,510
K0383 Luminaris Color HiGreen Fluorescein qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0384 Luminaris Color HiGreen Fluorescein qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0391 Luminaris Color HiGreen qPCR Master Mix 250 x 20 µL rxns 1,355
K0392 Luminaris Color HiGreen qPCR Master Mix 500 x 20 µL rxns 2,510
K0393 Luminaris Color HiGreen qPCR Master Mix 1,250 x 20 µL rxns 5,744
K0394 Luminaris Color HiGreen qPCR Master Mix 5,000 x 20 µL rxns 16,034
K0441 TranscriptAid™ T7 High Yield Transcription Kit 50 react 2,325
K0512 Genomic DNA Purification Kit 100 preps 595
K0513 Silica Bead DNA Gel Extraction Kit 100 preps 541
K0621 DecaLabel™ DNA Labeling Kit 10 react 541
K0622 DecaLabel™ DNA Labeling Kit 30 react 1,352
K0651 Biotin DecaLabel™ DNA Labeling Kit 10 react 919
K0652 Biotin DecaLabel™ DNA Labeling Kit 30 react 2,271
K0661 Biotin Chromogenic Detection Kit 10 react 865
K0662 Biotin Chromogenic Detection Kit 30 react 1,947
K1071 DreamTaq™ PCR Master Mix (2X) 200 react 541
K1072 DreamTaq™ PCR Master Mix (2X) 1000 react 2,163
K1081 DreamTaq™ Green PCR Master Mix (2X) 200 react 541
K1082 DreamTaq™ Green PCR Master Mix (2X) 1000 react 2,163
K1091 Fast Tissue-to-PCR Kit 200 react 2,271
K1213 InsTAclone™ PCR Cloning Kit 10 react 649
K1214 InsTAclone™ PCR Cloning Kit 30 react 1,406
K1231 CloneJET™ PCR Cloning Kit 20 react 1,146
K1232 CloneJET™ PCR Cloning Kit 40 react 2,163
K1422 Rapid DNA Ligation Kit 50 react 1,179
K1423 Rapid DNA Ligation Kit 150 react 2,358
K1611 First Strand cDNA Synthesis Kit 20 react 595
K1612 First Strand cDNA Synthesis Kit 100 react 2,379
K1621 RevertAid™ First Strand cDNA Synthesis Kit 20 react 433
K1622 RevertAid™ First Strand cDNA Synthesis Kit 100 react 1,730
K1631 RevertAid™ H Minus First Strand cDNA Synthesis Kit 20 react 757
K1632 RevertAid™ H Minus First Strand cDNA Synthesis Kit 100 react 3,028
K1641 Maxima® First Strand cDNA Synthesis Kit 50 react 2,704
K1642 Maxima® First Strand cDNA Synthesis Kit 200 react 8,111
K1651 RevertAid™ Premium First Strand cDNA Synthesis Kit 20 react 1,049
K1652 RevertAid™ Premium First Strand cDNA Synthesis Kit 100 react 4,196
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文献和实验过程是第一天seeding,第二天换low serun的opti-MEM加lipofactamin,Renilla,和我的质粒过夜。第三天加compound,incubate一个小时以后加PMA,PHA,在incubate6个小时以后测量。Empty vector的读数和NF-kB的读数差很远,我想Transfection还是成功的。但是加了PMA和PHA的和不加的读数几乎没有区别。加了compound的和不加的也没有区别。 raw ratio 都在15左右。请高手赐教,不胜
The Split Luciferase Complementation Assay
- and carboxy-terminal fragments of Renilla luciferase, are transiently expressed in protoplasts. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase
相关专题 在调控位点分析中,Luciferase主要采用的方法为:片段缺失、定点突变 ,流程如下所示。 图1 片段缺失分析 位点流程图 图2 定点突变分析位点流程图 荧光素酶报告基因检测法是转录调控研究中十分重要的实验手段。但其结果往往存在一定的误差。未解决这一问题,现普遍采用双荧光素酶报告基因检测法。在双荧光素酶报告基因测试中,将萤火虫荧光素酶作为实验报告基因,海肾荧光素酶作为对照报告
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