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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
2-10年
- 英文名:
Cell Signaling Technology
- 库存:
1000
- 供应商:
CST
- CAS号:
Polyclonal Antibody
- 规格:
100 ul
美国 Cell Signaling Technology(CST)是最知名的和老牌的信号转导公司之一,专注于信号转导产品的提供和研究。产品具有种类多、质量好、价格低、引用文献多等优点,是您进行信号转导研究不可多得的帮手!
提供特色的信号传导用磷酸化检测抗体、ELISA检测试剂盒、活性的重组激酶等药筛产品。涉及的细胞信号通路有: 1 染色质调控与乙酰化信号通路 2 MAP激酶信号网络 3 SAPK/JNK信号通路 4 细胞凋亡与Caspase信号网络 5 AKT信号网络 6 转录与翻译调控 7 蛋白激酶C PKC 信号网络 8 周期调控与DNA损伤信号通路 9 细胞骨架调控 10 白细胞信号传导,等等。
主要产品:
抗体类:提供超过1300种磷酸化和非磷酸化抗体,具有最多的磷酸化抗体,同时提供sample kit和phosphoPlus Ab kit
ELISA试剂盒:PathScan Sandwich ELISA Kit系列产品是检测蛋白磷酸化水平的ELISA试剂盒,灵敏度高,操作简单
WB用蛋白Marker:CST的Biotinylated Protein Ladder Detection Pack产品包括有10个条带、大小在10-200Kda之间的生物素标记蛋白标准和HRP藕联的抗生物素二抗(anti-biotin antibody, HRP-linked),有大小两种包装可选
kinase assay kit:试剂盒组成合理全面,非同位素操作,应用起来简单方便。
buffer类:共有6个buffer类,是进行普通WB或磷酸化WB检测的必须辅助试剂,用于标本蛋白的制备。
激酶类:多种形式的蛋白激酶,可以用于药物筛选
药物筛选试剂盒:高通量、针对激酶的药物筛选
The genomic era has yielded a wealth of new genes for which function and inter-relationships remain unknown. At the same time there is an increasing appreciation of the limits of what can be learned from gene expression profiling, and of the need to examine protein expression and activity. Activation-state-specific antibodies have proven to be a convenient and reliable set of tools in the new post-genomic "research toolbox". Cell Signaling Technology, Inc. (CST) is committed to developing innovative new research tools such as these to help define the mechanisms underlying cell function and disease, thereby broadly accelerating progress in biomedical research and medicine.
CST strives to make novel antibodies of the highest possible quality by producing them in-house and rigorously characterizing them with respect to specificity and sensitivity. Production begins with extensive purification (typically by both protein A and peptide affinity chromatography) followed by testing by our staff scientists on a wide range of applications, including ELISA, western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry and flow cytometry. Technical support is provided by the very scientists who make a given antibody and know it best. CST scientists are also responsible for creating most of the cell signaling reference material, including pathways, kinase trees and kinase disease associations that we offer our customers in the last chapter of this catalog and on our website.
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文献和实验【求助】caspase-3抗体与激活型caspase-3抗体
环佩从容 要做下一步实验啦,这两个抗体选哪个呀,目前研究有没有区分哪一个更好点啊 ronaldo_zj 楼主您好。 1. 楼主需要知道Pro caspase-3以及Cleaved caspase-3的功能以及检测手段,不是说哪个好那个不好的问题,而是根据你的课题需要检测何种片段的问题。请楼主先具体查阅关于caspase活化的文献后再定好实验计划; 2. 目前多数研究均认为Cleaved
olivial 最近想做caspase-3的WB,但关于caspase-3的活化有一个问题一直没弄明白,想请教。 在没受刺激前,细胞内的caspase-3以procaspase的形式存在,当受到内外源性刺激时,被活化成异二聚体,该异二聚体由p12和p17两个亚基组成。但procaspase是怎样变成异二聚体的呢?是self-cleavage,还是上游信号如caspase-8、9裂解的? selenium
bin1986 最近在做凋亡,在用凯基的caspase-3分光光度法试剂盒检测 细胞经过与药物孵育24小时后检测 用酶标仪检测,OD值在0.1-0.3之间,有一定浓度依赖,高浓度下caspase-3的活化程度有差不多4 问题在于,我用BCA蛋白定量得到的蛋白浓度时30多ug/ul,我加了有250ug,如果按照说明书的话,这个OD值应该在1左右,而我的最高才0.3.另外我的control孔(没加药)与我最低
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