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- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) 抗体
抗原
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
别名
E-Cad, E-cadherin, XB-cadherin, XBcad, XTCAD-1, b-cadherin, cadherin-1, cdh1-a, cdhc-A, cdhp, hjmd, l-cam, lcam, pcad, uvo, uvomorulin, xb-cad, xcdh1, Arc1, AA960649, E-cad, Ecad, L-CAM, UVO, Um, Arc- ... show more
适用
其他选择 兔, 小鼠, 大鼠, 猪, 牛, 马, 人
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 Western Blotting (WB), 流式细胞分析技术(FACS), 免疫组织化学石蜡技术(IHC-P), 免疫萤光石蜡技术(IF-P)
Pubmed 找到1个引用
规格 100 μL
发货至 中国 (更改)
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) 抗体产品细节
免疫原 KLH conjugated synthetic peptide derived from human E-cadherin
亚型 IgG
纯化方法 Purified by Protein A.
目标详细情况
别名 E Cadherin
背景 Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function.
分子量 20/80kDa
基因ID 999
使用细节
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) 抗体应用备注 WB(1:100-500)
FCM(1:100-500)
IHC-P(1:100-500)
IF(IHC-P)(1:100-500)
限制 仅限研究用
贮存及处理
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20 °C for 12 months.
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文献和实验Tracking the Transport of E-Cadherin to and From the Plasma Membrane
, it is also a key occurrence in many diseases, including cancers of epithelial origin E-cadherin is a central component of adherens junctions (AJs), which act as structural and signaling hubs in epithelial cells that oppose EMT. The loss of E-cadherin
- Dilution 1:25- Antigen retrieval method: HIER (pressure cooking) in EDTA- Staining pattern: Cell membrane staining (apical cell membrane is negative)- E-cadherin is a form of calcium-dependent cell adhesion molecule expressed in most epithelial cells
mammary epithelial cells. They presented evidence that siRNAs targeting CpG islands (regions of methylation) in the E-cadherin promoter increased methylation in MCF7 breast-cancer cells. The researchers showed this was an RNAi sequence-specific event
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