- 询价
- Abcam
- 中国/美国/德国
- xy10335-RP01
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
E-selectin / SELE / CD62E / ELAM1抗体
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, human cell-derived, recombinant human CD62E / E-selectin ( rh CD62E ; Catalog#10335-H08H ; NP_000441.2 ; Met 1- Pro 556 ). Total IgG was purified by Protein A affinity chromatography
E-selectin / SELE / CD62E / ELAM1 Antibody Background
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed at relatively high density on the surface of cultured endothelial cells that have been activated by proinflammatory cytokines, such as IL-1β, TNF-α, or bacterial endotoxin. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. This adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
X2 （Chi square method）也是一种好适度测验的方法，可以用来检验我们的实验结果和理论值有无差异，其公式为： X2 =Σ（d2 /e） e: 为预期值，即理论值； d：预期值和观察值或实验值之差。例如我们做了以下两组豌豆测交：查卡方表，一对性状，自由度N=2-1，X2 =10，P2 =2，0.05 X2 值的计算（紫花（Rr）×白花（rr
E-Z 96 X-press Plasmid Vacuum Protocol
well plate inside the manifold base; 3) Place the top plate of manifold over the base, the deep well plate or waste collection tray now should be positioned under the E-Z 96 X-press Plate. Seal the unused wells of E-Z 96 Xpress Plate with sealing
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