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- 2025年07月10日
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文献和实验Preparation of Carboxypeptidase Y and Its Properties
Activation of carboxypeptidase Y 3. Separation and purification of carboxypeptidase Y ⑴ Separation carboxypeptidase Y from above-mentioned solution by various methods. ⑵ Assay of protein purification before purification. ⑶ Assay
Hematoxylin and Eosin (HE) Staining
Reagents Stains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions (make fresh
Hematoxylin and Eosin Staining
O x1 Ammonia solution 10 dips (x1) (changes the stain from purple to blue) Running H2 O 5 min 80% EtOH x1 Eosin 15" 95% EtOH x2 100% EtOH Xylene 3 min x2 Mounting Wipe off the xylene
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