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文献和实验serum (FBS;HyClone, Ogden, UT) and L -glutamine (Gibco/BRL). 2. Phosphate-buffered saline (PBS) containing 0.5 M ethylenediaminetetraacetic acid (EDTA). 3. Solution of trypsin (0.25%) containing 1 mM EDTA (Gibco/BRL). 4. Western lysis buffer
Southern Hybridization Protocols
-phosphate buffer pH 7.2 7% (w/v) SDS 2% (w/v) BSA 1 mM EDTA pH 8.0 Preparation of solution (not easy to prepare therefore follow the recipe): Prepare 0.3 M Sodium-phosphate buffer by adding 40.2g NaH2PO4 to 400 ml sterile distilled water
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, UK). 3. Red Ponceau solution (1×): 0.2% red Ponceau powder, 3% trichloroaceticacid (TCA) in water. Store at room temperature. 2.6. Agarose Gel Electrophoresis to Detect Full Length APC (~300 kDa) 1. Laemmli sample buffer (4×): 250 mM
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