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常温，避光
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H3N2 (A/Brisbane/10/ 2007) HA / Hemagglutinin抗体
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, recombinant Cynomolgus IL-13 (rC IL-13; Catalog#11057-C07H; ABG75889.1; Ser 21-Asn 132). IL-13 specific IgG was purified by Cynomolgus IL-13 affinity chromatography.
H3N2 (A/Brisbane/10/ 2007) HA / Hemagglutinin抗体 Background
Interleukin 13 (IL-13) is a single-chain cytokine which belongs to the IL13/IL4 family. In humans, IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL13 inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. Meanwhile, IL-13 synergizes with IL2 in regulating IFN-gamma synthesis. IL-13 binds to a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL13 Rα1 and IL13 Rα2), and exerts its effects through signal transducer and activator of transcription 6 (STAT6), like IL-4. Accordingly, IL-13 may be critical in regulating inflammatory and immune responses as a central mediator in various cell types.
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文献和实验人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
luozhifeng339 请教各位达达，我准备做EMSA，如果做SUPER-SHIFT的话，该选择原装抗体还是分装的抗体呢，盼不吝赐教！ wanglinling 看到一篇EMSA的文章，不知道里面有没有回答你的问题哈，不过英文的值得阅读哈。 GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure
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