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- Omega
- Omega
- TQ2501-01
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详见说明书
- 保质期：
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- 英文名：
M-MLV first strand cDNA Synthesis Kit(20)
- 库存：
电询
- 供应商：
上海古朵生物有限公司
品名: M-MLV first strand cDNA Synthesis Kit(20)(PCR与RT-PCR相关) M-MLV first strand cDNA Synthesis Kit:M-MLV第一链cDNA合成试剂盒 货号:TQ2501-01 品牌：Omega
M-MLV first strand cDNA Synthesis Kit(20)(PCR与-PCRT相关) 产品特性与优点
1.是纯化产物最可靠、最快速的试剂盒
2.操作简单，每次都能获得理想的效果
3.性价比最高
本产品仅用于科研实验，不用于临床诊断！
M-MLV first strand cDNA Synthesis Kit(20)(PCR与-PCRT相关)相关产品介绍:
| 10mM dNTPs | |
| TQAC135 | dNTP solution,10mM（4x250ul) |
| TQAC136 | dNTP solution,25mM（4x250ul) |
| 100mM dNTP each | |
| TQAC131 | dATP solution,100mM（250ul) |
| TQAC132 | dcTP solution,100mM（250ul) |
| TQAC133 | dGTP solution,100mM（250ul) |
| TQAC134 | dTTP solution,100mM（250ul) |
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文献和实验Protocol for First-strand cDNA Synthesis
℃for 10 minutes and then at 37℃for 60 minutes.Stop the reaction by heating at 70℃for 10 minutes. Chill on ice.NoteThe synthesized cDNA can be amplified by the PCR (see Protocols for PCR using Taq and Pfu DNA Polymerases) without intermediate phenol
of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
cDNA Synthesis from MOLT-4 Cells
second strand yield. 11. Add 0.5 ml PN buffer from Qiegen Nucleotide Removal Kit to the remaining reaction, and mix it ,load it into the spin column, and follow the pr℃edures comimg with the kit to purify the double strand DNA. 12. Elute the cDNA
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