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联硕生物
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文献和实验for this purpose. ::Chaotrophe: urea and thiourea::Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP)::Detergent: CHAPS, triton x-100::Ampholytes::Protease inhibitors Some researchers also add tris buffer.Chaotrophe.Urea
addition of contaminating proteases and an increased complication in the preparation of the sample, we don't bother. We always ultracentrifuge (30mins at 100 000 x g at 20oC) our samples before we use them anyway and in general find it quick, easy and non
to the bottom, top and middle of the sample tube. If the density and viscosity of the medium are known, as well as the density of a given particle, then the time needed to completely sediement a particle can be determined by the formula: T = ((D-L)/(D+L))*(N/(d(g-p)S
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