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- 文献和实验
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- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
Gag-p24抗体
Antibody Type : Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
克隆号 : 9B6C2B7
抗体宿主 : Mouse IgG1
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HIV-1 Gag-p24 (HIV-1 Gag-p24; Catalog#11695-V08E; ACI05538.1; Pro 133-Leu 363). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Gag-p24抗体 Background
Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses. Gag protein from HIV-1 is a polyprotein, which, during viral maturation, is cleaved to release matrix p17, core p24 and nucleocapsid proteins. Capsid protein p24 forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is dissassembled soon after virion entry. The p24 antigen contains epitopes that prime helper CD4 T-cells, which have been demonstrated to be protective and it can elicit lymphocyte proliferation. p24 is likely to be an integral part of any multicomponent HIV vaccine.
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文献和实验p24 p2p7p1p6 p55 Gag CTL epitopes Gag/Pol CTL epitopes protease RT Integrase Pol Vif Tat Vpr, Rev Vpu gp160 Env CTL epitopes Nef HIV CTL Epitope Maps p17 p24 p2p7p1p6, Protease, RT Integrase Rev, gp160 Nef HIV CTL Epitope
或至少有一条env带和一条gag(p55,p24,p18)带或至少有一条env带和一条gag带,一条pol带或至少2条env带。 (2)、阴性:无病毒特异带。 (3)、可疑:一条gag带和一条pol带或只有gag带或只有pol带。
1、ELISA :对gp41 HIV 1型抗体的检测 是标准的筛选方法,敏感性可达99.5%,但特异性并不高。该法的阳性者中仅13%为真正的HIV者,因此,属筛选试验。 2、蛋白印迹(Western印迹)法:是最常用的证实实验。若检出所有3个HIV主要基因gag、po1、env产物的抗体时为阳性,可确诊为HIV感染。 3、HIV1型抗原检测 由于血中p24抗原浓度低且形成了免疫复合物,p24抗原检测阳性率不如抗体阳性率高。在HIVl型感染的无症状
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