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- xy11639-MM12
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
OLFM4 / GW112抗体
Antibody Type : Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID : 12
抗体宿主 : Mouse IgG1
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human OLFM4 / GW112 (rh OLFM4 / GW112; Catalog#11639-H08H; NP_006409.3; Met 1-Gln 510).
OLFM4 / GW112抗体 Background
Olfactomedin-4, also known as G-CSF-stimulated clone 1 protein, Antiapoptotic protein GW112, and OLFM4, is a secreted protein which contains oneolfactomedin-like domain. OLFM4 is expressed during myeloid lineage development. It is strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. OLFM4 is highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. It is also expressed at high levels in stomach cancer and colon cancer tissues. Inhibition of ROS or the ERK pathway remarkably decreased G-CSF-induced OLFM4 expression. OLFM4 is an antiapoptotic factor that promotes tumor growth. OLFM4 promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. OLFM4 also facilitates cell adhesion. Induction of OLFM4 in cancer cells was reported to have a novel antiapoptotic action via binding to the potent apoptosis inducer GRIM-19. The human OLFM4 is also thought to be a useful marker for early myeloid development.
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文献和实验.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation
。 2、临床标本检测结果 将胃镜检查患者的血清以1：100稀释，用上述实验条件建立的ELISA间接法进行，Hp抗体（ELISA－HpAb）检测，同时将同一患者胃粘膜进行Hp分离培养（HpC）和Hp尿素酶测定（HpUT），以资比较（表5）。 表4 5份阳性血清标本进行ELISA的批间误差试验结果 标本号 分批检测阳性血清的OD值 X SD
切片Giemsa染色 93.7 94.8 94.3 血清学Hp抗体测定 92.2 88.9 90.6 Giemsa染色法检测敏感性、特异性和准确度较尿素酶试验、血清学Hp抗体测定略高，但三者之间比较差异无显著性（P＞0.05，表1）。男性Hp感染率为71.6％（154/215）。女性为68.6％（116/169）。两者比较无显著性差异（P＞0.05）。Hp感染率随着年龄增加而增加，各年龄组感染率分别为：20岁～39岁59.6％（81/136），40岁～59岁70.9％（112/158），60岁以上85
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