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- xy11695-R002
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
HIV Type 1 Gag-P24抗体
Antibody Type : Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
克隆号 : 002
抗体宿主 : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant HIV-1 Gag-P24 ( rv Gag-P24 ; Catalog#11695-V08E ; ACI05538.1 ; Pro 133 - Leu 363 )
HIV Type 1 Gag-P24抗体 Background
Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses. Gag protein from HIV-1 is a polyprotein, which, during viral maturation, is cleaved to release matrix p17, core p24 and nucleocapsid proteins. Capsid protein p24 forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is dissassembled soon after virion entry. The p24 antigen contains epitopes that prime helper CD4 T-cells, which have been demonstrated to be protective and it can elicit lymphocyte proliferation. p24 is likely to be an integral part of any multicomponent HIV vaccine.
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文献和实验The Design, Production, and Validation of an Anti-HIV Type 1 Ribozyme
(3 ,4 ) with an immune response evident, namely antibodies to env and p24 as well as HIV specific cytotoxic T-lymphocytes (5 ).
(PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.
以上阴性对照的OD值＞0.1，应重复实验。）。样品OD值＜临界值者为HIV抗体阴性。 （4）、正常情况下，阳性对照孔的OD值≥0.80（若1孔阳性对照的OD值＜0.8应舍弃，若有2孔或2孔以上阳性对照的OD值＜0.8，应重复实验。样品OD值≥临界值者为HIV抗体阳性。 3、HIV测定（蛋白印迹法）结果的判定方法 根据卫生部1990(2)号文件的规定，蛋白印迹法的判断标准为： （1）、阳性：至少有一条env(gp160，gp120，gp41)带和一条pol(p65，p51，p32)带
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